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Effect of ethanol consumption on ACh- and SNP-induced relaxation. The concentration–response curves for ACh and sodium nitroprusside (SNP) were obtained in endothelium-intact and -denuded aortic rings, respectively. ACh-induced relaxation was determined in the absence or after 30-min incubation with L-NAME (100 μmol l−1). *Compared to control and ethanol-treated groups; **compared to control, ethanol-treated and control+L-NAME (P<0.05; ANOVA). L-NAME, NG-nitro-L-arginine methyl ester.
