Table 1.
Diversity of the CDR3 libraries
| CAM phenotypeb | |||||
| Initial diversitya | ++ | + | - | final diversityc | |
| H3-5 | 1.4e8 | 15/20 | 3/20 | 2/20 | 1.1e8 |
| H3-6 | 2.7e7 | 20/20 | 0/20 | 0/20 | 2.7e7 |
| H3-7 | 9.2e7 | 16/20 | 3/20 | 1/20 | 7.4e7 |
| H3-8 | 5.0e6 | 14/20 | 4/20 | 2/20 | 3.5e6 |
| H3-9 | 2.4e8 | 16/20 | 1/20 | 3/20 | 1.9e8 |
| H3-10 | 1.0e7 | 10/20 | 6/20 | 4/20 | 5.0e6 |
| H3-11 | 2.2e7 | 9/12 | 1/12 | 2/12 | 1.7e7 |
| H3-12 | 1.0e7 | 15/20 | 2/20 | 3/20 | 7.5e6 |
| H3-13 | 2.3e7 | 8/12 | 3/12 | 1/12 | 1.5e7 |
| H3-14 | 1.0e7 | 12/20 | 4/20 | 4/20 | 6.0e6 |
| H3-15 | 2.1e7 | 8/12 | 3/12 | 1/12 | 1.4e7 |
| H3-16 | 1.0e7 | 12/20 | 2/20 | 6/20 | 6.0e6 |
| H3-17 | 1.1e7 | 12/20 | 4/20 | 4/20 | 6.6e6 |
| K3-9 | 3.6e6 | 11/16 | 1/16 | 4/16 | 2.5e6 |
| K3-10 | 4.4e6 | 10/16 | 1/16 | 5/16 | 2.8e6 |
| L3-9 | 1.7e7 | 11/20 | 6/20 | 3/20 | 9.4e6 |
| L3-10 | 1.5e7 | 10/20 | 9/20 | 1/20 | 7.5e6 |
| L3-11 | 1.8e7 | 15/20 | 3/20 | 2/20 | 1.4e7 |
a Initial diversity of the library cloned as fusion with CAT and selected on ampicillin. This is the number of clones obtained after transformation.
b Between 12 and 20 clones from the transformation were checked on CAM plates. Plates were incubated for 16 h at 37°C and colony size estimated. Columns ++,+ and - give, respectively, the fraction of clones that grew normally, gave tiny colonies, and did not grow at all.
c Diversity of the libraries selected on CAM. The diversity is estimated from column "Initial Diversity" and "CAM phenotype" by assuming that the final diversity is close to (Initial Diversity) × (CAM phenotype ++). The real diversity may be a higher since some of the clones noted + in column "CAM phenotype" may be present in low abundance.