Table 2.

Sequences of some anti-tubulin scFvs

	VH CDR3		VL CDR3
Name	Sequence	length	Sequence	length	frequencya	yieldb	WBc	IFd
Round 2
C12C	SSITIFGGGMDV	12	HSREVHRTF	9	1/5	19
E12C	SGGNTFDY	8	QQYYRKPWT	9	1/5	53
F1C	GNADGGENWELFDK	14	QLYQNTLWT	9	2/5	52
H6C	SSITIFGGGMDV	12	QQNWTSPLS	9	1/5	nd
Round 3
2C1C	RGRDY	5	QQYNTSPFS	9	1/6	8.6	+	-
2E11C	GRNVLNY	7	QQNSSSPRFT	10	2/6	8.7	+	-
2F12C	GRRALGN	7	QQYNTSPFS	9	1/6	45	+	+
2G4C	GRRALGN	7	LTWSMRSAI	9	1/6	15	+	+
2G9C	GRRALGN	7	LTTENSVYRLV	11	1/6	50	+	-

Sequences of the CDR3s of the best positive clones in an ELISA using cytoplasmically expressed scFv from the 2^nd (5 clones) and the 3^rd (6 clones) round of selection (Table 2). ^a Frequency of apparition of the scFv among sequenced clones of the same round. ^b Yield: mg of scFv purified from 1 liter of cells grown in a flask (OD₆₀₀ = 5). ^c WB: detection of tubulin in brain extracts by Western blot. ^d IF: + means that the scFv is able to reveal microtubule network by Immunofluorescence (Fig. 8). The sequences of the clones 2C1C, 2E11C, 2F12C, 2G4C and 2G9C have been submitted to the EMBL database and their accession numbers are respectively AM886280, AM886281, AM886282, AM886283 and AM886284.