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ELISA detection of soluble and insoluble antigens of boiled C. parvum oocysts. Oocysts were control, boiled for 10 minutes, or boiled 10 minutes and separated into insoluble fractions and soluble fractions by centrifugation. Oocysts were then detected using anti-Cryptosporidium polyclonal antibody (1.25 μg/ml). Error bars represent one standard deviation. Signal to noise values above 2 are considered positive.
