Figure 5.

Sequence dependence of pol κ termination within mononucleotide alleles. Phosphorimager quantitation of percent synthesis termination for 30 min reactions at pH 7.0 and 30°C are shown for (A). the A₁₁ allele (mean of five experiments); (B) the A₁₁ allele in the complementary sequence context (mean of five experiments); and (C) the G₁₀ allele (mean of four experiments). Insets show representative phosphoimager scans of primer extension products, with arrows indicating reaction time (5–30 min). Boxed areas indicate the beginning and end of each microsatellite allele. Sequence below graph indicates sequence context of microsatellite and arrows indicate direction of synthesis. –pol, no polymerase control, showing that the dark band at the 3′ end of the A_11C allele is an impurity in the primer and not a pause site.