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. 2008 Jan 4;36(2):e11. doi: 10.1093/nar/gkm1075

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© 2008 The Author(s)

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/2.0/uk/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 3. — Rank of mean and standard deviation relations of the technical replicate microarrays after preprocessing. The red dots depict the running median estimator (window-width 10%). Plotted are two 100:0 (blood:placenta) replicates in the Barnes data set, which are separately located at two HumanRef-8 BeadChips. (A) VST- transformed and quantile-normalized; (B) log2-transformed and quantile-normalized; (C) Regular VSN-processed. (D) VSN-techReplicate method performed VSN only using the pair of technical replicates. Note: for Figure A–C, all six pairs of samples were preprocessed together, although only the replicates of the 100:0 group were plotted.