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. 2007 Nov 27;36(2):489–500. doi: 10.1093/nar/gkm1066

Figure 6.

Figure 6.

DSB repair-mediated instability of the (GAA•TTC)n sequence is independent of the length of the intervening sequence at the center of the repeat tract. (A) GAA-70-spacer construct containing a 28 bp spacer in the XbaI site of GAA-70X, such that BamHI would cut in the center of the spacer (indicated by the black box). (B) DSB repair at the BamHI site in GAA-70-spacer construct produced a dramatic rise in instability. Note that the (GAA•TTC)n sequence was equally unstable when transformed into E. coli MM28 [wild-type (WT)] and its isogenic recA mutant strain, M152. All error bars represent +/−2 SEM derived from triplicate experiments. (C) DSB repair at the center of the (GAA•TTC)n sequence results in the preferential deletion of approximately half of the total repeat length. The residual tract lengths of the (GAA•TTC)n sequence are shown (as a percentage of full-length) after transformation of BamHI-linearized GAA-70-spacer vector in E. coli MM28 (WT) and M152 (recA).