Figure 5.

Denaturing PAGE (20%) analysis of RNA-40 digestion in 30 min by 0.2, 1 or 3 U of RNase H with 2 µM [γ-³²P]-labeled RNA-40, 0.01 µM C6 in RNase H buffer at 37°C. Lanes 1–3, control asODN, C6 and cleaved C6 with 0.2 U RNase H; Lanes 4–6, control asODN, C6 and cleaved C6 with 1 U RNase H; Lanes 7–9, control asODN, C6 and cleaved C6 with 3 U RNase H. C6 was photocleaved with UV light (355 nm, 36 mW/cm²) for 10 min. The columns show the ratio of degraded RNA to total RNA, data from two or three independent trials.