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. 2007 Dec 15;36(3):793–802. doi: 10.1093/nar/gkm1093

Figure 1.

Figure 1.

RNA polymerase I purification and identification of phosphorylation sites. (A) Pol I purification scheme. See Supplementary Figure S1 for the extended scheme, including an additional cation exchange chromatography step. (B) Coomassie staining of purified Pol I [C] and phosphoprotein staining of the same gel [P] using phosphostain ProQ Diamond (Invitrogen). Three proteins of an eIF3 subcomplex co-purified with Pol I, namely Rpg1p (1*), Nip1p (2*) and Prt1p (3*). (C) Scheme of the strategy used for the identification of phosphorylation sites from purified Pol I.