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. 2007 Dec 20;36(3):1037–1049. doi: 10.1093/nar/gkm1120

Figure 3.

Figure 3.

Effect of RNA length on RNA export in the transcription-coupled system (III). (A) Diagram of the DNA constructs. One to three copies of a 100-nt DNA fragment from the DHFR gene were cloned between the promoter and terminator of the U1 gene. The U1ΔSm gene was also used as a control. (B) Export of the transcripts produced from the microinjected plasmids harboring 1–3 copies of a 100-nt DNA fragment from the DHFR gene between the promoter and terminator of the U1 gene was analyzed as in Figure 1B. The U1ΔSm gene was also used as a control. (C) Quantitation of RNA export inhibition by CTE WT from the experiments like that shown in B. (D) Export of the transcripts produced from the microinjected plasmids as in B was analyzed as in Figure 1D. The U1ΔSm gene was also used as a control. (E) Quantitation of RNA export inhibition by PHAXΔNES from the experiments like that shown in D.