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. 2007 Dec 20;36(3):1037–1049. doi: 10.1093/nar/gkm1120

Figure 5.

Figure 5.

Effect of transcription from an mRNA transcription unit on RNA export. (A) Diagram of the DNA constructs. Human β-globin cDNA fragments of various lengths were cloned between the CMV promoter and the poly (A) signal from the bovine growth hormone (BGH) gene. The U1ΔSm gene was also used as a control. (B) Export of the transcripts produced from the microinjected plasmids harboring the cDNA fragments of various lengths (50–360 nt) between the CMV promoter and the BGH poly (A) signal was analyzed as in Figure 1B. Only the polyadenylated transcripts were visualized (see ‘Materials and Methods’ section). (C) The transcript from the plasmid harboring the U1ΔSm gene was analyzed as a control as in Figure 1B. (D) Quantitation of B and C.