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Binding titration for Pfu-Pol (black circles) and Mac-Pol (white circles). The polymerases were added to Hex-GCCCGCGGGAUATCGGCCCTTA (6 nM) and the fluorescence anisotropy measured (the same number of measurements were carried out for both polymerases; in some cases the white circles obscure the black). The titration was carried out three times to give a KD of 8.5 ± 1.3 nM for Pfu-Pol and 9.7 ± 1.6 nM for Mac-Pol.
