Table 1.
Effect of overexpression of recombinant wild-type or mutant Y. pestis topoisomerase I with substitutions at Gly122 on the viability of E. coli JD5
| Subsitution at Gly122 | Induced/non-induced relative viability |
|---|---|
| none | 0.17 ± 0.11 |
| Ser | 5.2 × 10−4 ± 1.8 × 10−5 |
| Ala | 0.093 ± 0.015 |
| Asp | 0.19 ± 0.02 |
| Asn | 0.17 ± 0.10 |
| Cys | 0.030 ± 0.004 |
| Glu | 0.092 ± 0.040 |
| Gln | 0.11 ± 0.06 |
| His | 0.14 ± 0.02 |
| Ile | 0.086 ± 0.033 |
| Phe | 0.075 ± 0.035 |
| Thr | 0.087 ± 0.018 |
| Trp | 0.13 ± 0.05 |
| Tyr | 0.088 ± 0.016 |
Relative viability (RV) was measured by the ratio of the viable colonies obtained after induction of the BAD promoter directing the expression of recombinant YTOP with 0.2% arabinose for 2 h in comparison with viable colonies from the culture not treated with arabinose. The results shown represent the average and standard deviation from at least three measurements.