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. 2007 Dec 15;36(3):839–848. doi: 10.1093/nar/gkm874

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© 2007 The Author(s)

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/2.0/uk/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 2. — Non-denaturing PAGE analysis of RNAs (A) with 5 mM MgCl₂ and (B) without MgCl₂. The 19-mer was mixed with varying ratios of 35-mer or 35 -uucg and heterodimer formation was assessed by native gel electrophoresis. The lanes in each gel contain: (1) 0.45 nmol 19-mer; (2) 0.45 nmol 19-mer and 0.15 nmol 35-mer; (3) 0.45 nmol 19-mer and 0.30 nmol 35-mer; (4) 0.45 nmol 19-mer and 0.45 nmol 35-mer and (5) 0.45 nmol 35-mer. In lanes (6–10), 35 -uucg was used instead of 35-mer.