Figure 7.

In vivo localization of MLE and MLE derivatives. (A) Drosophila SF4 cells were transiently transfected with either MLE or the indicated MLE derivatives, all fused to GFP at the C-terminus. Localization of the fusion proteins was visualized by using anti-GFP antibodies (αGFP) and the X territory was marked with anti-MSL1 antibodies (αMSL1). The DNA was counterstained with Hoechst 33258 (DNA). (B) Quantitative analysis of the experiment. Clear and selective X chromosomal staining was scored as positive (‘+’), whereas a clear enrichment of GFP at sites of MSL1-binding over a diffuse nuclear staining was scored as intermediate (‘+/−’).