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. 2007 Dec 20;36(3):1009–1016. doi: 10.1093/nar/gkm1087

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© 2007 The Author(s)

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/2.0/uk/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 6. — ATP-dependent rescue of AZTMP terminated DNA synthesis. (A) Primer rescue reactions using 10 nM P_30-AZTMP/T₅₀ were performed with 5 mM ATP, 0.02 U pyrophosphatase and 100 μM dCTP, dGTP, TTP and ddATP. Lane C, no enzyme added. (B) Quantification of (A) by densitometry. The percentage of the remaining unextended primer is shown.