Figure 4.

Effect of cortical injection of C(92-96) and mAb NGF30 on the number (A) and on the size (B) of VAChT-IR sites in the hindlimb and in the parietal I and II cortices. Note in A the loss of cholinergic boutons (VAChT-IR), which is more prominent with the TrkA antagonist C(92-96) and is related to the distance from the injection site; B illustrates the shrinkage of the cholinergic boutons after the administration of both compounds. The drugs were injected into the hindlimb cortex as indicated in Materials and Methods. Twelve 8,500-μm² sections were digitized from five consecutive sections in each cortical area of each rat (five animals per group). The number of VAChT-IR sites (A) in 1,000 μm² is expressed as the mean ± SEM. The size of VAChT-IR sites (B) (in square micrometers) is expressed as the mean ± SEM. HL, hindlimb; Par II, parietal II. ∗, P < 0.05 vs. each control peptide area; ∗∗, P < 0.01 vs. each control peptide area.