Table 1.

Summary of spectroscopic properties for roGFP1-R mutants

Varianta	ID	δb	Φc	ɛd (M−1 cm−1)
S147C/Q204C	roGFP1	6.5	0.64	20,200
S147C/Q204C/F223R	R1	5.6	0.60	24,100
S147C/Q204C/N149K	R3	7.0	0.58	20,100
S147C/Q204C/F223R/S202K	R7	6.2	0.64	25,700
S147C/Q204C/F223R/K41D	R8	7.5	0.42	12,600
S147C/Q204C/N149K/Y151D	R9	7.2	0.64	15,200
S147C/Q204C/F223R/S202K/K41D	R10	6.2	0.65	22,500
S147C/Q204C/N149K/F223R	R11	5.4	0.60	18,700
S147C/Q204C/N149K/F223R/S202K	R12	5.6	0.62	23,100
S147C/Q204C/N149K/F223R/S202K/Y151D	R14	5.6	0.60	20,700

^a Variants also contain the two phenotypically neutral substitutions, C48S and Q80R.

^b The dynamic range value (δ) is the maximum observed δ-fold change in excitation peak ratio.

^c The fluorescence quantum yield for emission with excitation at 400 nm.

^d Extinction coefficients for absorbance at 400 nm.