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. 2006 Jan;15(1):45–57. doi: 10.1110/ps.051734306

Table 5.

Calculated vs. experimental rate enhancements for reduction with DTT

Calculated ΔpKa Calculated rate
Ionic strength (mM) C147 C204 C147 C204 Enhancements avg.a Observedb
A. roGFP1-R8
    50 0.033 0.074 0.928 0.844 0.886 0.857
    100 0.020 0.054 0.954 0.883 0.919 0.886
    150 0.015 0.043 0.967 0.905 0.936 0.873
    250 0.009 0.032 0.979 0.929 0.954 0.924
    350 0.007 0.025 0.984 0.943 0.964 0.928
CC:c 0.88 0.91 0.90
B. roGFP1-R7
    50 –0.426 –0.656 2.666 4.525 3.595 3.107
    100 –0.366 –0.593 2.323 3.918 3.120 2.402
    150 –0.333 –0.558 2.154 3.613 2.883 1.975
    250 –0.295 –0.515 1.971 3.277 2.624 1.768
    350 –0.271 –0.489 1.868 3.083 2.475 1.630
CC:c 0.99 0.99 0.99
C. R7 with C-terminal residues removed
    50 –0.421 –0.596 2.636 3.947 3.291 3.107
    100 –0.346 –0.511 2.217 3.246 2.731 2.402
    150 –0.304 –0.463 2.014 2.901 2.458 1.975
    250 –0.255 –0.403 1.798 2.531 2.164 1.768
    350 –0.224 –0.365 1.677 2.319 1.998 1.630
CC:c 0.99 0.99 0.99

a From ΔpKa, calculated using the program DelPhi (see Materials and Methods).

b From comparison of roGFP1 and full-length roGFP1-R7 or roGFP1-R8 experimental rate constants using DTT (see Materials and Methods).

c Correlation coefficient of calculated to experimental rate enhancements.