Figure 2.
[1H-15N]HSQC NMR spectra of WT-IL4 (A) and C24T-IL4 (B) in 25 mM d3-acetic acid, 90% H2O, and 10% D2O (pH 5.1) at 25°C. The protein concentration was ~2 mM in both cases, and spectra were run at a 1H frequency of 500 MHz.
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