Figure 5. CCK8-S increases mIPSCs in TTX.
CCK application increases miniature IPSCs (mIPSCs) recorded under whole-cell voltage clamp in the presence of 1 μM tetrodotoxin. A) Representative 30-sec traces of mIPSCs before (upper trace) and in the same cell in CCK8-S (lower trace). Calib. = 20 pA, 1 sec. Group data (n=5 cells) show that mIPSC frequency (B1) and amplitude (B2) are transiently increased during application of CCK8-S, and return to baseline levels after wash-out of the peptide (black circles). The increase in mIPSCs is blocked by the CCK2 receptor antagonist LY225910 (2μM; grey circles, n=4 cells).