Figure 8.

Effect of scanning mutagenesis of highly conserved prosequence residues on human cathepsin S propeptide function; “XnpA” means that amino acid X in position n of the propeptide is substituted by Ala. Propeptide mutants have been expressed, purified, and characterized as published by Kreusch et al. (2000). Renaturation rates (left ordinate, •) are from Pietschmann et al. (2002). Inhibiton constants (right ordinate, □) and red shift of the endogenous tryptophan fluorescence, standing for compactness of tertiary structure (abscissa), are from Schilling et al. (2001).