Figure 3.

Chemical exchange line broadening in WT and mutant RNases H. Backbone amide ¹⁵N R _ex at 300 K, 14.1 T for WT ecRNH, WT ttRNH, iG80b ecRNH, and dG85 ttRNH are shown. Residues with significant chemical exchange line broadening (R _ex ≥ 2.5 sec⁻¹) are indicated by open circles. Vertical dashed lines indicate the sites of mutation. Secondary structural elements are diagrammed at the top of the figure. Data for WT ttRNH are from Butterwick et al. (2004).