Figure 2.

HHP mediated-solubilization yield of IBs (1 mg/mL) incubated under 200 MPa at 25°C for 24 h in the refolding buffer (50 mM Tris-Cl at pH 8.0, 150 mM NaCl, 1 mM EDTA, 2 mM DTT, 6 mM GSSG, 0.05% sodium azide) containing the various additives. The numbers below each column represent the buffer formulation as follows: (1) buffer alone; (2) 1 M urea; (3) 2 M urea; (4) 2.5 M glycerol; (5) 0.5 M arginine; (6) 0.1 mM Tween 20; (7) 0.5 mM Triton X-100. After decompression, samples were centrifuged (12,000g for 10 min) to remove insoluble aggregates and the supernatants were used for the total protein assay as described in the Materials and Methods in detail. The error bars represent the standard deviation for triplicate incubated samples.