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Functional binding assay of the recovered soluble GNBPs from their respective IBs (1 mg/mL) by HHP (200 MPa, 25°C, 24 h) in the refolding buffer containing 0.5 M arginine. The binding assay was performed as described in the Materials and Methods in detail using the various microbial cell wall components, β-1,3-glucan (A), LPS (B), chitin (C), cellulose (D), and peptidoglycan (E). GNBP1 (lane 1); GNBP2 (lane 2); GNBP3 (lane 3); and BSA as a negative control (lane 4).
