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Metal ion effects on the refolding kinetics of BLA. (a) Refolding kinetics in the presence of various concentrations of Ca2+. (b) Refolding kinetics in the presence of 30 mM of the different metal ions, with the exception of Zn2+ (10 mM). For the apo-form, the refolding is monitored in the presence of 1 mM EDTA. The kinetics deduced from the fitting procedure are hardly distinguishable from the data. As a consequence, they are not shown.
