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Summary of the solubility test
Solubility was estimated from the protein concentration remaining in the supernatant at the indicated pH, and classified into four classes (ranging from - to+++). Each pH was tested under a low salt (0 M NaCl) and high salt (300 mM NaCl) condition. SDS-PAGE gels used to estimate the solubility are provided in the supplemental material (Supplement 2).
