Figure 2.

The oligosphere cells cultured on ornithine-coated coverslips in the presence of B104CM were positive for vimentin (a), A2B5 (b), and PDGFRα (c) but negative for O4 (d). In the presence of PDGF alone for 7 DIV, the oligosphere cells were largely bipolar or tripolar. There were also round cells (arrowheads) and a few multiprocess-bearing cells (arrows) (e). In the presence of 0.5% FBS for 2 DIV, all cells were O4+ (f) and many cells were O1+ (g). At 7 DIV, cells were MBP+ (h). In the presence of 10% FBS, almost all cells were positive for GFAP (i) and A2B5+ (j). Incubation of the culture with BrdUrd for 20 h indicated that the majority of cells were labeled in the nuclei (yellow in k). All cells were A2B5+ (red in k). (l) A single sphere plated on ornithine-coated dish in the presence of B104CM for 24 h shows that bipolar cells migrated out of the sphere. The nuclei of cells in (f–h) were stained with 4′,6-diamidino-2-phenylindole (DAPI). (Bar = 100 μm.)