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. 2006 May;15(5):1063–1075. doi: 10.1110/ps.052004006

Figure 2.

Figure 2.

Aggregates of 20kPEG-GCSF are soluble. SEC-HPLC chromatograms of (A) GCSF and (B) 20kPEG-GCSF at t = 0 and t = 72 h of incubation at 37°C, pH 6.9. Monomer retention time of GCSF was ∼12 min, and that of 20kPEG-GCSF was ∼8.5 min. The high molecular weight material (HMW) in the 20kPEG-GCSF sample after 48 h of incubation eluted in the void volume (6–7 min). (C) Quantitation of the amount of soluble aggregates (HMW in the void volume) at each time point relative to time zero for GCSF (filled triangles) and 20kPEG-GCSF (open triangles).