Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2006 May;15(5):1063–1075. doi: 10.1110/ps.052004006

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright © 2006 The Protein Society

PMC Copyright notice

Figure 3. — Similar extent of oligomeric and covalent character in GCSF and 20kPEG-GCSF aggregates. (A) Silver-stained SDS-PAGE gels of solubilized pellets of GCSF and GCSF + free 20kPEG, and isolated soluble aggregate of 20kPEG-GCSF. The gels were run both nonreduced (left panel) and reduced (right panel). The monomer and dimer positions for GCSF (closed arrow) and 20kPEG-GCSF (open block arrow) are indicated. (B) Denatured SEC chromatograms are shown for solubilized GCSF pellet (top panel) and isolated 20kPEG-GCSF soluble aggregates (bottom panel). Samples were either reduced and alkylated (“Red”), or run as alkylated but nonreduced (“NR”). The percent covalent character for each sample from this analysis has been indicated.