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. 2006 May;15(5):1063–1075. doi: 10.1110/ps.052004006

Figure 5.

Figure 5.

20kPEG-GCSF can rescue GCSF precipitation. (A) GCSF was incubated for 48 h at pH 6.9, 37°C either alone (black bar), or with 1:1 20kPEG-GCSF (gray bar), 2:1 20kPEG-GCSF (hatched bar), or free 20k PEG (white bar). The starting GCSF concentration was 5 mg/mL, and protein turbidity was monitored by absorbance at 700 nm. (B) GCSF was co-incubated with 1:1 20kPEG-GCSF as described above, and supernatants analyzed by SEC are shown at t = 0, t = 60 h and t = 120 h. The peaks corresponding to GCSF monomer, PEG-GCSF monomer, and soluble oligomers are shown.