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Favorable solvation of PEG-GCSF by water. Solvation differences between GCSF + free 20kPEG and 20kPEG-GCSF. Chromatograms of GCSF (A) and 20kPEG-GCSF (B) before (−octanol) and after overnight incubation in a 10% octanol–water mixture. Post incubation, the phases were separated and the aqueous phase was analyzed by SEC to determine extent of protein partitioning away from the aqueous phase.
