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Similar principles in copper induced GCSF aggregation. SEC chromatograms of supernatant of 0.5 mg/mL GCSF solution (A) or 20kPEG-GCSF solution (B) after incubation with 0.1 mM copper (II) chloride for 4 h, in comparison to the no-copper (t = 0) control. Protein elution in the void volume (“Soluble aggregates”) and buffer peaks are indicated. (C) Quantitation of percent of GCSF (filled triangles) or 20kPEG-GCSF (open triangles) that was monomeric during the incubation period.
