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ESI mass spectra of mixtures of pseudo-wild type b* and barnase: (A) 20 μM b* and 25 μM bn and (B) 25 μM b* and 20 μM bn. ESI solution is 200 mM aqueous ammonium bicarbonate, 1% (v/v) glycerol (pH 7.2). An identical region including the (bn + 6H) 6+ and (b* + 5H) 5+ molecular ions is inset in both spectra.
