Figure 4.

The RNA binding region of SRP68. (Top panel) Mild digestion of fragments 68a (left) and 68a′ (right) with V8 protease followed by the separation of the fragments on 15% polyacrylamide Tricine gels; the migration distances of 68a and fragments 68-1 to 68-7 are indicated. The arrowhead marks the 68-7 fragment of 6.5 kDa generated by the primary V8 protease cut. (Lower left panel) Comigration of fragments 68a (black bars) and 68a-1 (gray bars) with human SRP RNA (black line) during sucrose gradient centrifugation. (Lower right panel) Collective distribution of the proteolytic fragments 68-2 to 68-7 (gray bars) generated by V8 protease. As in the lower left panel, the black line indicates the mobility of human SRP RNA.