Figure 5.
Interactions of the protein binding site of SRP68 with fragments of SRP72. (A) The 68c polypeptide was mixed with SRP72 fragments 72a′, 72b′, 72c, or 72d and analyzed by sucrose gradient centrifugation. Fraction aliquots were analyzed by SDS-PAGE. (B) Testing the interaction between 68c′ and 72b′. (C) Testing for binding between the RNA binding domain of SRP68 (fragment 68a bound to human SRP RNA, indicated as 68a-R) and 72b′. Gradient fractions 3–15 are indicated in top of each panel.