Figure 1.

Database search for the identification of conserved domains and cysteines. (A) Domain prediction in Rel WT protein from M. smegmatis and in its deleted versions. (B) Deletion in the Rel full-length protein based on the prediction of the domains in Rel WT. The molecular masses of the constructed fragments are also mentioned. The region at the end of each bar represents the Hexa-histidine tag. Positions of the start and the end of the domains have been mentioned. The locations of the cysteines present in all of the constructs have been marked by filled circles (•). (C) Sequence alignment of Rel protein sequences from M. tuberculosis, M. leprae, M. smegmatis, and E. coli. Only the C-terminal region with three conserved cysteines is shown.