Eight percent of SDS–polyacrylamide gel showing the purification of Rel WT protein (lanes a-c) and its deleted versions. (Lanes d-f) Rel dACT, (lanes g-i) Rel NTD. Lanes a, d, and g represent the crude cell lysate of the respective proteins, whereas lanes c, f, and i represent the purified proteins. Lanes b, e, and h are column wash, showing no bands due to the large volume (100 column volumes) and low protein concentration.