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. 2006 Jun;15(6):1449–1464. doi: 10.1110/ps.062117006

Figure 8.

Figure 8.

Western analysis of the Rel WT protein expressed in E. coli BL21 (DE3). (A) Rel WT protein probed with anti-Rel antibodies. Lanes a–c represent the crude cell lysate overexpressing Rel protein and treated as mentioned in Materials and Methods. Lanes d and e are the protein (control) run without DTT. (B) Rel WT protein probed with anti-His tag antibodies. Lanes a and b represent the crude cell lysate treated as mentioned in Materials and Methods. Lanes c and d represent the protein run in the presence and the absence of DTT, respectively, and act as controls.