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Examples of measurement couplings of lactose from coupled 1H-13C HSQC spectra. (A) Lactose in phosphate buffer; (B) lactose in the absence of protein in 3%C12E5; (C) lactose in the presence of Galectin-3 C251-CRD in 3% C12E5 10:1 ratio; (D) lactose in the presence of Galectin-3 S-propyl-C251-CRD in 3%C12E5 10:1 ratio. All spectra were collected at 298 k with a proton resonance frequency of 800 MHz. All samples were prepared using 75 mM phosphate buffer (pH 7.4).
