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Functional analysis of Bodipy FL prazosin accumulation in HeLa cells transiently expressing wild-type ABCG2 and all 19 ABCG2 mutants. Cells were incubated with 0.5 μM Bodipy FL prazosin for 30 min at 37°C and kept on ice until flow cytometric analysis. The percent of accumulation was calculated by dividing the mean fluorescence values obtained for all the variants by the mean fluorescence value of the pTM1 vector and multiplying by 100.
