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Time-dependent emission spectra (λem = 295 nm) of the FRET system of IAEDANS-αAW9F with crystallin donors: (A) αB wild-type, (B) αB wild-type + αAW9F, (C) αAW9F. The sample solutions were kept at 37°C, and measurements were made each hour from 0 to 5 h (curves 1–6).
