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Time-dependent emission spectra (λem = 295 nm) of the FRET system of IAEDANS-αAW9F with crystallin donors: wild-type and R116C αA-crystallin, wild-type and R120G αB-crystallin, and wild-type and Q155* βB2-crystallin. The sample solutions of equal amounts of donor and acceptor proteins (0.1 mg/mL) were kept at either 37°C (A) or 45°C (B), and measurements were made each hour from 0 to 5 h (curves 1–6).
