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. 2006 Aug;15(8):1977–1986. doi: 10.1110/ps.062113906

Figure 4.

Figure 4.

Time dependence of subunit exchange at different steps of the catalytic cycle. Mixtures of 25 nM EII-AF488 and EII-AF633 were prepared in a C10E5-containing buffer and placed for up to 40 min at 30°C in the absence (open circles) or the presence (solid circles) of 1 mM mannitol. To prephosphorylate the separate samples of EII-AF488 and EII-AF633, the phosphorylation components (see Materials and Methods) were added and the samples were placed for 10 min at 30°C to allow complete phosphorylation of EIImtl, after which the samples were mixed and placed for up to 40 min at 30°C (squares). Addition of 1 mM perseitol was performed right after the mixing of the prephosphorylated samples (stars). The amount maximal amount of cross-correlation was calculated as described in Materials and Methods. The error bar reflects the typical error of three to six individual measurements.