Figure 3.

Concentration-dependent perturbation of αIIbβ3 solution conformation as measured by the fractional change in sedimentation coefficient, S/S₀, versus the molar excess of echistatin variant, rEch:αIIbβ3. The dotted line denotes S/S₀ = 1.0. Symbols as follows: rEch (1–49) M28L D26A (black triangles), rEch (1–49) M28L D26E (open triangles), rEch (1–49) M28L R24A (gray diamonds), rEch (1–49) M28L R24K (black diamonds), rEch (1–49) M28L (open circles), rEch (1–49) M28L D27W (black hexagons), rEch (1–49) M28L D27F (gray hexagons). The black and gray lines were obtained by fitting the data with full-length echistatin and the R24A mutant, respectively, to a hyperbolic inhibition model (Hantgan et al. 1999).