Figure 4.

Effects of recombinant 30-kDa γ-module (γ 148–411) on the distribution of αIIbβ3 sedimenting species, g(S) versus S, as determined with time-derivative software (DCDT+; Philo1997). Each panel depicts the distributions obtained with ligand-free integrin (open circles; left axis), αIIbβ3 + γ 148–411 (black squares; right axis), and γ 148–411 alone (gray triangles; right axis). Continuous lines were obtained by fitting the resultant distribution functions to a one- or two-species model, as required. (A) Resting αIIbβ3 + γ 148–411; (B) primed αIIbβ3^* + γ 148–411.