Figure 6.

Protein synthesis and signaling to the soma are not required for the acute potentiation of ACh release induced by NT-3. (A) Pretreatment with anisomycin (40 μM) or with cycloheximide (10 μM) for 1 h before bath application of NT-3 does not prevent rapid potentiation of ACh release at spontaneously formed synapses. Changes in the SSC frequency with time after the onset of NT-3 treatment. Each data point represents the mean ± SEM of five experiments. ∗, P < 0.01. (B) Potentiation of ACh release at the distal axonal fragments. The axon was transected in the vicinity of the cell body (Insert) and the SSC frequency was measured. Bath application of NT-3 (marked by arrow) resulted in a characteristically rapid potentiation of ACh release from the distal axonal fragments innervating muscles. Each data point is a mean ± SEM of five experiments. ∗, P < 0.01.