Figure 3.
Effects of octanol and butanol on the intrinsic fluorescence of PKCδ C1A. Quenching of PKCδ C1A fluorescence by octanol (filled square) and butanol (filled circle) is plotted after subtracting the data for nonspecific quenching of tryptophan (open symbols; see text). Curved lines represent nonlinear least square fits to the logistic equation (see Materials and Methods). F and Fmax are the average fluorescence intensities at 348–350 nm in the presence and absence of alcohol. Control experiments in which identical additions of DMSO were made to PKCδ C1A in buffer did not cause any significant fluorescence quenching (data not shown).