Table 4.
Summary of protocols and costs
| Technical stage | Stage presence or duration | ||||
| PathVysion | PharmDx | INFORM | CISH | In-house | |
| Deparaffinisation and rehydration | 40 min | 33 min | Overnight in the BenchMark | 25 min | 20 min |
| Pretreatment | 67 min | 31 min | 21 min | 60 min | |
| Digestion | 26 min | 16 min | 16 min | No | |
| Fixation | 20 min | No | No | Fixation, blocking, incubation with primary and secondary antibodies and detection, all performed in NexES 90 min | |
| Dehydration | No | 6 min | 10 min | ||
| Drying | Yes | Yes | Yes | ||
| Denaturation | 5 min | 5 min | 5 min | ||
| Hybridisation | Overnight | Overnight | Overnight | ||
| Washing | 4 min | 20 min | 4 min | 11 min | Washing, haematoxylin counterstain (15 sec), dehydration and mounting, 12 min |
| Dehydration | No | 6 min | 3 min | No | |
| Drying | 30 min | Fast | Fast | No | |
| DAPI staining | Yes | Yes | Yes | No | |
| Detection | No | No | No | 147 min | |
| Total duration | Overnight + 3 h 12 min | Overnight + 1 h 57 min | Overnight + 7 min | Overnight + 3 h 55 min | 3 h 2 min |
| Price (€ per test) | 131 | 85 | 75 | 56 | 4 |
CISH, chromogenic in situ hybridisation; DAPI, 4,6-diamidino-2-phenylindole.