Fig. 1.

Adherence of parasitized HbS and HbC erythrocytes to HMVECs. (a) Adherence of parasitized erythrocytes to HMVECs under semistatic conditions. Results from AS, SS, AC, and CC erythrocytes were normalized to those from AA erythrocytes run in parallel. Mean percentages are indicated by horizontal bars. Results were obtained from three parasite lines (3D7, FVO, and 7G8) and multiple blood donors (17AA, 9AS, 1AS/β-thalassemia, 3SS, 2AC, and 2CC) (not all combinations tested). The mean (± SEM) number of parasitized AA erythrocytes per HMVEC was 3.7 ± 0.5. (b) Adherence of P. falciparum 7G8-infected erythrocytes to HMVECs under physiologic flow conditions. Results from three AS erythrocyte donors were normalized to those from five AA erythrocyte donors run in parallel and are expressed as mean percentages (not all combinations tested). The mean (± SEM) number of parasitized AA erythrocytes at 5% parasitemia was 179 ± 48/mm². (c) Adherence of erythrocytes infected with naturally occurring P. falciparum isolates to HMVECs under semistatic conditions. Results from four AS erythrocyte donors were normalized to those from four AA erythrocyte donors run in parallel and are expressed as mean percentages. Data are from 13 parasite isolates (not all combinations tested). The mean (± SEM) number of parasitized AA erythrocytes per HMVEC was 3.7 ± 0.5.